O-1: Suitability of Epididymal and Testicular Ultrasonography and Computerized Image Analysis for Assessment of Current and Future Semen Quality in The Ram

Background: Breeding soundness evaluation (BSE) is the primary assessment for determining reproductive potential of male animals. This method, however, cannot be used to evaluate semen frequently or to predict future semen quality. Computerized analysis of ultrasonographic images provides information on histophysiological changes in male reproductive organs.We hypothesized that: 1. semen parameters would correlate with ultrasonographic characteristics of the distal region (cauda) of the epididymis and 2. testicular ultrasound images and/or circulating testosterone (T) concentration would predict future semen quality in the ram. Materials and Methods: Six adult rams underwent BSE and scrotal ultrasonography approximately 60 days apart (average duration of the spermatogenic cycle) both during the breeding (December and February) and nonbreeding (June and August) seasons. Results: An inverse correlation was found between pixel intensity (numerical pixel values) of the epididymes and percentage of sperm in semen with normal morphology (r=-0.46, p<0.05). Pixel heterogeneity (standard deviation of pixel values) correlated negatively with percentage of sperm with normal morphology (r=-0.42, p<0.05) and directly with percentage of spermatozoa with abnormal tails (r=0.43, p<0.05). Pixel heterogeneity of testicular parenchyma obtained approximately 60 days prior to semen evaluation inversely correlated with percentage of sperm with normal morphology (r=-0.73, p<0.01) and sperm progressive motility (r=- 0.76, p<0.01), and directly with percentage of sperm with abnormal tails (r=0.72, p<0.01) and loose heads (r=0.79, p<0.01). Conclusion: We concluded that scrotal ultrasonography combined with computer-assisted analyses of epididymal and testicular echotexture in the ram was a valuable method for determining certain current and future semen parameters, respectively.